Isolation of β-globin related genes from a human cosmid library.
April 1981
Article
volume 13, issue 3 pp 227-237.
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A human gene library was constructed using an improved cloning technique for cosmid vectors. Human placental DNA was partially digested with restriction endonuclease MboI; size-fractionated and ligated to BamHI-cut and phosphatase-treated cosmid vector pJB8. After packaging in lambda phage particles, the recombinant DNA was transduced into Escherichia coli 1400 or HB101 followed by selection on ampicillin for recombinant E. coli. 150 000 recombinant-DNA-containing colonies were screened for the presence of the human beta-globin related genes. Five recombinants were isolated containing the human beta-globin locus and encompassing approx. 70 kb of human DNA.
Keywords
- Human
- Support, Non-U.S. Gov't
- Cloning, Molecular
- 0 (Genetic Vectors)
- Genes, Structural
- 0 (DNA, Recombinant)
- DNA, Recombinant
- Genetic Vectors
- 9004-22-2 (Globins)
- Bacteriophage lambda/genetics
- Globins/genetics
Automatically Extracted Terms
- filter
- cosmid
- fragment
- colony
- vector
- chromosomal
- coshg
- nitrocellulose filters
- library
- globin
- vector dna
- packaging
- placental dna
- cosmid dna
- nitrocellulose
- procedure
- plating
- ligated
- probe
- plate
