Isolation and characterization of the human collagen α1(I)-like gene from a cosmid library.
January 1994
Article
volume 10, issue 8 pp 1981-1994.
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We have isolated a human collagen alpha 1(I)-like gene from a cosmid library. The clone which contains 37kb of human DNA has been shown to contain this gene by DNA sequencing, hybrid arrest and hybrid selection assays and Northern blot hybridizations. The collagen gene sequence extends through most of the cloned DNA and must, therefore, be at least 35kb in length.
Keywords
- 0 (Plasmids)
- 9007-49-2 (dna)
- Human
- Support, Non-U.S. Gov't
- Base Sequence
- 0 (RNA, Messenger)
- RNA, Messenger/genetics
- *Genes, Structural
- Nucleic Acid Hybridization
- Transcription, Genetic
- DNA Restriction Enzymes
- EC 3.1.21 (DNA Restriction Enzymes)
- 9007-34-5 (Collagen)
- Collagen/*genetics
- *DNA/*genetics
- *Plasmids
Automatically Extracted Terms
- collagen
- sequence
- fragment
- chicken
- clone
- cosh col .1
- region
- ppstl
- ecori
- research
- cosmid
- cosh col.l
- probe
- hybridized
- hybridization
- dna sequence
- chain
- procollagen
- collagen gene
- col.l
