Cloning of a cDNA encoding the smallest neurofilament protein from the rat
January 1985
Article
volume 825 pp 398-404.
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We have cloned a cDNA coding for the smallest rat neurofilament protein. The cDNA is 861 nucleotides long coding for 287 amino acids from the internal alpha-helical region and the carboxy-terminal tail domain of the neurofilament protein. Comparison of the porcine, mouse and rat neurofilament protein sequences shows that the protein is highly conserved (greater than 93% identity). Blot analysis indicates that the cDNA is derived from a single neurofilament gene that codes for two different poly(A)+ mRNA species.
Keywords
- Animals
- 9007-49-2 (dna)
- Support, Non-U.S. Gov't
- Amino Acid Sequence
- Cloning, Molecular
- Base Sequence
- 0 (RNA, Messenger)
- RNA, Messenger/genetics
- Rats
- DNA/genetics
- Genes, Structural
- Transcription, Genetic
- Molecular Weight
- 0 (Oligodeoxyribonucleotides)
- Nerve Tissue Proteins/*genetics
- 0 (Intermediate Filament Proteins)
- 0 (Nerve Tissue Proteins)
- 0 (Neurofilament Proteins)
- Intermediate Filament Proteins/*genetics
- Neurofilament Proteins
- Oligodeoxyribonucleotides/diagnostic use
