http://hdl.handle.net/1765/2403

Progress towards construction of a total restriction fragment map of a human chromosome.


Article
volume 15 pp 1363-1375.
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We present an approach to the construction of an overlapping restriction fragment map of a single human chromosome. A genomic cosmid library genome was constructed from a mouse-human hybrid cell line containing chromosome 17 as its only human genetic component. Cosmids containing human inserts were isolated by hybridisation to a human Alu sequence. DNAs from ninety-six randomly chosen cosmids were digested with either EcoRI or HindIII, end-labelled with 35S-dATP and analysed using agarose gel electrophoresis. Comparison of the restriction fragment patterns revealed two pairs of overlapping clones, that were confirmed by cross-hybridization of the overlapping fragments. The two pairs of cosmids both mapped to human chromosome 17, as shown by hybridization to a panel of somatic cell hybrids. These data demonstrate that the generation of an overlapping cosmid map along a human chromosome is feasible, representing an intermediate step towards the complete sequencing of a human chromosome.



Keywords


Automatically Extracted Terms
  • cosmid
  • chromosome
  • fragment
  • ecori
  • sequence
  • research
  • hindiii
  • genome
  • acids research
  • clone
  • number
  • library
  • cosmid library
  • chromosome 17
  • analysis
  • marker
  • agarose
  • vector
  • restriction
  • hybridised


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