http://hdl.handle.net/1765/2423

Ectopic expression of Thy-1 in the kidneys of transgenic mice induces functional and proliferative abnormalities.


Article
volume 51 pp 21-31.
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Hybrid human--mouse Thy-1.1 genes were injected into pronuclei of Thy-1.2 mice to produce transgenic animals. A hybrid gene composed of the 5' part of the mouse Thy-1.1 gene combined with the 3' human untranslated regions was expressed abnormally in the kidney podocytes, which resulted in severe protein-uria and subsequent death in several founder mice. A hybrid Thy-1 gene composed of the human coding region with the 5' and 3' flanking regions of the mouse gene was expressed abnormally in a different part of the kidney (the tubular epithelia), which resulted in a proliferative kidney disorder. In addition, a neoplasm was found in the brain of one of these mice. These results show that the Thy-1 protein can play an important role in the activation, proliferation, and differentiation of many different cell types.



Keywords


Automatically Extracted Terms
  • thy-l
  • kidney
  • figure
  • expression
  • mouse
  • antibody
  • level
  • brain
  • transgene
  • podocyte
  • animal
  • serum
  • proteinuria
  • arrow
  • tissue
  • thy-l gene
  • figure 2 b
  • cell lines
  • sequence
  • morri


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