The transcriptional unit of the murine Thy-1 gene unit: Differential distribution of transcription initiation sites in brain.
January 1988
Article
volume 8 pp 3847-3856.
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Structural analysis of the mouse Thy-1.2 gene has shown that the major promoter of the gene is characterized by a tissue-specific DNase I-hypersensitive site and is located within a methylation-free island. The gene is regulated at the transcriptional level, and steady-state mRNA analysis reveals that the previously reported exon Ib contributes at most 5% of the total mRNA. The major promoter uses several transcription initiation sites within a region of 100 base pairs. The frequency of usage of these sites in brain is markedly different from that in other tissues.
Keywords
- Animals
- Support, Non-U.S. Gov't
- Mice
- Molecular Sequence Data
- Base Sequence
- *Transcription, Genetic
- *Genes, Structural
- Exons
- Blotting, Northern
- Mice, Inbred Strains
- 0 (Chromatin)
- EC 3.1.21.1 (Deoxyribonuclease I)
- DNA Restriction Enzymes
- EC 3.1.21 (DNA Restriction Enzymes)
- 0 (Antigens, Surface)
- 0 (Antigens, Thy-1)
- Antigens, Surface/*genetics
- Antigens, Thy-1
- Chromatin/ultrastructure
- Deoxyribonuclease I
- Nucleotide Mapping
- Brain/*metabolism
