Hypersensitive site 4 of the human β-globin locus control region.
January 1991
Article
| Related Files |
|---|
View PDF Version
(eur_grosveld_9074.pdf, 1.8MB) |
The Locus Control Region (LCR) of the human beta globin gene domain is defined by four erythroid-specific DNasel hypersensitive sites (HSS) located upstream of this multigene cluster. The LCR confers copy number dependent high levels of erythroid specific expression to a linked transgene, independent of the site of integration. To assess the role of the individual hypersensitive sites of the LCR, we have localized HSS4 to a 280bp fragment that is functional both in murine erythroleukaemia (MEL) cells and in transgenic mice. This fragment coincides with the major area of hypersensitivity 'in vivo' and contains a number of DNasel footprints. Bandshift analysis shows that these footprints correspond to binding sites for the erythroid specific proteins GATA1 and NF-E2 and a number of ubiquitous proteins, including jun/fos, Sp1 and TEF2.
- Animals
- 9007-49-2 (dna)
- Human
- Support, Non-U.S. Gov't
- Mice
- Molecular Sequence Data
- Base Sequence
- Hela Cells
- Blotting, Southern
- Binding Sites
- Mice, Transgenic
- Chromosome Mapping
- DNA Fingerprinting
- Electrophoresis, Agar Gel
- Deoxyribonuclease I/metabolism
- EC 3.1.21.1 (Deoxyribonuclease I)
- DNA/analysis
- *Regulatory Sequences, Nucleic Acid
- 9004-22-2 (Globins)
- Globins/*genetics
- fragment
- globin
- hss 4
- binding
- expression
- mel cells
- grosveld
- hypersensitive
- hss 2
- level
- protein
- oligonucleotide
- factor
- acids research
- probe
- number
- transgene
- region
- oligo
- dnasei
