The minimal requirements for activity in transgenic mice of hypersensitive site 3 of the β globin locus control region.
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Proper expression of the human beta-like globin genes is completely dependent on the presence of the locus control region or LCR, a region containing four DNase hypersensitive sites (HS1-4) situated 5' to the structural genes. Linkage of the LCR to a transgene results in copy number-dependent transcription, independent of the site of integration in the host genome. We have analysed a small region of the LCR (HS3) in transgenic animals to determine the minimal interactions that are required for this property. The results show that a specific combination of a G-rich sequence flanked on each side by one binding site for the transcription factor GATA1 is essential to obtain position-independent expression of a linked beta globin gene in erythroid cells. The overall transcriptional activity of HS3 is achieved through synergy with other combinations of similar binding sites.
- 9007-49-2 (dna)
- Support, Non-U.S. Gov't
- Molecular Sequence Data
- Base Sequence
- 0 (DNA-Binding Proteins)
- Mice, Transgenic
- DNA-Binding Proteins/metabolism
- 0 (Transcription Factors)
- Zinc Fingers
- Transcription Factors/metabolism
- *Regulatory Sequences, Nucleic Acid
- 9004-22-2 (Globins)
- 125267-48-3 (erythroid-specific DNA-binding factor)