Functional Analysis of the Human Neurofilament Light Chain Gene Promoter
January 1993
Article
volume 21 pp 455-461.
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We have carried out a structural and functional analysis on the human NF-L (H-NF-L) gene. It contains a methylation-free island, spanning the 5' flanking sequences and the first exon and a number of neuronal-specific DNase I hypersensitive sites have been identified in the upstream region as well as within the body of the gene. Analysis in cell lines and transgenic mice using a combination of these sites has revealed the presence of a conserved element(s) between -300bp and -190bp which is required for neuronal-specific expression.
Keywords
- Animals
- Cell Line
- 9007-49-2 (dna)
- Human
- Support, Non-U.S. Gov't
- Mice
- Molecular Sequence Data
- Transfection
- Base Sequence
- Hela Cells
- Sequence Homology, Nucleic Acid
- Gene Expression
- Restriction Mapping
- Dna
- 0 (Chromatin)
- *Promoter Regions (Genetics)
- EC 3.1.21.1 (Deoxyribonuclease I)
- 0 (Neurofilament Proteins)
- 0 (neurofilament protein L)
- Chromatin/ultrastructure
- Deoxyribonuclease I
- Neurofilament Proteins/*genetics/metabolism
Automatically Extracted Terms
- promoter
- expression
- nf-l gene
- deletion
- figure
- transgene
- probe
- mouse
- control
- fragment
- hypersensitive
- sequence
- hypersensitive sites
- brain
- analysis
- region
- nf-l promoter deletion
- nf-l promoter deletions
- transfection
- level
