Estrogen-inducible and liver-specific expression of the chicken very low density apolipoprotein II gene locus in transgenic mice.
January 1993
Article
volume 21 pp 1629-1635.
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We have examined the chicken Very Low Density Apolipoprotein II (apoVLDL II) gene locus in transgenic mice. A DNA fragment composed of the transcribed region, 16 kb of 5' flanking and 400 bp of 3' flanking sequences contained all the information sufficient for estrogen-inducible, liver-specific expression of the apoVLDL II gene. The far-upstream region contains a Negative Regulating Element coinciding with a DNaseI-hypersensitive site at -11 kb. In transgenic mice, the NRE at -11 kb is used for downregulating the expression to a lower maximum level. The NRE might be used for modulating apoVLDL II gene expression, and may be involved in the rapid shut-down of the expression after hormone removal.
Keywords
- Animals
- Support, Non-U.S. Gov't
- Gene Expression Regulation
- Mice
- Molecular Sequence Data
- Base Sequence
- Regulatory Sequences, Nucleic Acid
- Chickens
- Mice, Transgenic
- Support, U.S. Gov't, P.H.S.
- Mice, Inbred C57BL
- Mice, Inbred CBA
- Organ Specificity
- EC 3.1.21.1 (Deoxyribonuclease I)
- Liver/metabolism
- Deoxyribonuclease I
- 0 (Estrogens)
- 0 (Lipoproteins, VLDL)
- 0 (apolipoprotein VLDL II)
- 0 (Apolipoproteins)
- Apolipoproteins/*genetics
- Estrogens/physiology
- Lipoproteins, VLDL/*genetics
Automatically Extracted Terms
- apovldl
- expression
- liver
- region
- chicken
- mouse
- fragment
- probe
- figure
- level
- transgene
- tissue
- transcription
- acids research
- -11 kb
- analysis
- promoter
- -11
- sequence
- locus
