Microinjection of Micrococcus luteus UV-endonuclease restores UV-induced unscheduled DNA synthesis in cells of 9 xeroderma pigmentosum complementation groups.
January 1985
Article
volume 150, issue 1-2 pp 99-105.
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The UV-induced unscheduled DNA synthesis (UDS) in cultured cells of excision-deficient xeroderma pigmentosum (XP) complementation groups A through I was assayed after injection of Micrococcus luteus UV-endonuclease using glass microneedles. In all complementation groups a restoration of the UV-induced UDS, in some cells to the repair-proficient human level, was observed. Another prokaryotic DNA-repair enzyme, T4 endonuclease V, restored the UV-induced UDS in a similar way after microinjection into XP cells. Since both enzymes specifically catalyse only the incision of UV-irradiated DNA, we conclude that this activity is impaired in cells of all 9 excision-deficient XP complementation groups tested.
Keywords
- 9007-49-2 (dna)
- Human
- Microinjections
- Support, Non-U.S. Gov't
- *DNA Repair
- Ultraviolet Rays
- Xeroderma Pigmentosum/enzymology/*genetics
- Genetic Complementation Test
- Cells, Cultured
- EC 3.1.- (Endodeoxyribonucleases)
- EC 3.2.2.- (N-Glycosyl Hydrolases)
- 0 (Multienzyme Complexes)
- *N-Glycosyl Hydrolases
- 81611-73-6 (UV endonuclease)
- DNA/biosynthesis/*radiation effects
- EC 3.1.22.3 (deoxyribonuclease V)
- Endodeoxyribonucleases/administration & dosage
- Micrococcus/enzymology
- Multienzyme Complexes/*administration & dosage
