Evolution and mutagenesis of the mammalian excision repair gene ERCC-1
January 1988
Article
| Related Files |
|---|
View PDF Version
(eur_hoeijmakers_9046.pdf, 1.6MB) |
The human DNA excision repair protein ERCC-1 exhibits homology to the yeast RADIO repair protein and its longer C-terminus displays similarity to parts of the E.coli repair proteins uvrA and uvrC. To study the evolution of this 'mosaic' ERCC-1 gene we have isolated the mouse homologue. Mouse ERCC-1 harbors the same pattern of homology with RAD10 and has a comparable C-terminal extension as its human equivalent. Mutation studies show that the strongly conserved C-terminus is essential in contrast to the less conserved N-terminus which is even dispensible. The mouse ERCC-1 amino acid sequence is compatible with a previously postulated nuclear location signal and DNA-binding domain. The ERCC-1 promoter harbors a region which is highly conserved in mouse and man. Since the ERCC-1 promoter is devoid of all classical promoter elements this region may be responsible for the low constitutive level of expression in all mouse tissues and stages of embryogenesis examined.
- Animals
- Cell Line
- 0 (Plasmids)
- Comparative Study
- Human
- Plasmids
- Support, Non-U.S. Gov't
- *DNA Repair
- 0 (Proteins)
- Amino Acid Sequence
- Cloning, Molecular
- Mice
- Molecular Sequence Data
- Proteins/*genetics
- Base Sequence
- Sequence Homology, Nucleic Acid
- *Genes, Structural
- Nucleic Acid Hybridization
- Saccharomyces cerevisiae/*genetics
- *Evolution
- DNA Replication
- Escherichia coli/genetics
- mouse
- protein
- mouse ercc
- sequence
- repair
- promoter
- research
- region
- figure
- domain
- level
- acids research
- homology
- nucleotide
- yeast
- probe
- position
- 43-3
- expression
- evolution
