Cloning and characterization of p52, the fifth subunit of the core of transcription/repair factor TFIIH.
January 1997
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TFIIH is a multiprotein factor involved in transcription and DNA repair and is implicated in DNA repair/transcription deficiency disorders such as xeroderma pigmentosum, Cockayne syndrome and trichothiodystrophy. Eight out of the nine genes encoding the subunits forming TFIIH have already been cloned. We report here the identification, cDNA cloning and gene structure of the 52 kDa polypeptide and its homology with the yeast counterpart TFB2. This protein, along with p89/XPB, p62, p44 and p34, forms the core of TFIIH. Moreover, using in vitro reconstituted transcription and nucleotide excision repair (NER) assays and microinjection experiments, we demonstrate that p52 is directly involved in both transcription and DNA repair mechanisms in vitro and in vivo.
- Human
- Microinjections
- Support, Non-U.S. Gov't
- *DNA Repair
- Amino Acid Sequence
- Cloning, Molecular
- Molecular Sequence Data
- Sequence Alignment
- Chromosome Mapping
- Blotting, Western
- 0 (Fungal Proteins)
- In Situ Hybridization, Fluorescence
- Transcription, Genetic
- *Transcription Factors, TFII
- 0 (Taf6 protein, Drosophila)
- 0 (Transcription Factors)
- 0 (Transcription Factors, TFII)
- 148710-81-0 (transcription factor TFIIH)
- Sequence Analysis
- 0 (Antibodies, Monoclonal)
- Antibodies, Monoclonal/immunology/pharmacology
- Chromosomes, Human, Pair 6
- Electrophoresis, Polyacrylamide Gel
- Fungal Proteins/chemistry
- Saccharomyces cerevisiae/chemistry
- Transcription Factors/*chemistry/genetics/isolation & purification/metabolism
