XAB2, a novel tetratricopeptide repeat protein, involved in transcription-coupled repair and transcription.
November 2000
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Nucleotide excision repair is a highly versatile DNA repair system responsible for elimination of a wide variety of lesions from the genome. It is comprised of two subpathways: transcription-coupled repair that accomplishes efficient removal of damage blocking transcription and global genome repair. Recently, the basic mechanism of global genome repair has emerged from biochemical studies. However, little is known about transcription-coupled repair in eukaryotes. Here we report the identification of a novel protein designated XAB2 (XPA-binding protein 2) that was identified by virtue of its ability to interact with XPA, a factor central to both nucleotide excision repair subpathways. The XAB2 protein of 855 amino acids consists mainly of 15 tetratricopeptide repeats. In addition to interacting with XPA, immunoprecipitation experiments demonstrated that a fraction of XAB2 is able to interact with the transcription-coupled repair-specific proteins CSA and CSB as well as RNA polymerase II. Furthermore, antibodies against XAB2 inhibited both transcription-coupled repair and transcription in vivo but not global genome repair when microinjected into living fibroblasts. These results indicate that XAB2 is a novel component involved in transcription-coupled repair and transcription.
- Animals
- Human
- Microinjections
- Support, Non-U.S. Gov't
- *DNA Repair
- Protein Binding
- Amino Acid Sequence
- Molecular Sequence Data
- Hela Cells
- 0 (DNA-Binding Proteins)
- *Transcription, Genetic
- Sequence Homology, Amino Acid
- 0 (Fungal Proteins)
- DNA-Binding Proteins/metabolism
- Fibroblasts/metabolism
- Gene Library
- 0 (Transcription Factors)
- EC 2.7.7.- (RNA Polymerase II)
- RNA Polymerase II/metabolism
- 0 (DNA, Complementary)
- Precipitin Tests
- Two-Hybrid System Techniques
- Cell Line, Transformed
- 0 (xeroderma pigmentosum group A complementing protein)
- DNA, Complementary/metabolism
- *Protozoan Proteins
- Amino Acid Motifs
- Cell Adhesion Molecules/metabolism
- Fungal Proteins/chemistry/metabolism
- Glutathione Transferase/metabolism
- Transcription Factors/chemistry/*physiology
- 0 (Cell Adhesion Molecules)
- 0 (HCNP protein, human)
- 0 (Protozoan Proteins)
- 0 (cell cohesion molecule, Dictyostelium)
- 0 (csb protein, Dictyostelium discoideum)
- EC 2.5.1.18 (Glutathione Transferase)
- xab 2
- protein
- rna polymerase ii
- transcription
- repair
- anti-xab
- polymerase
- rna synthesis
- synthesis
- antibody
- anti-xab 2fl
- sequence
- novel tpr protein
- fraction
- factor
- novel
- interaction
- genome repair
- dna repair
- antiserum
