The evaluation of a physical method for the quantification of inactivated poliovirus particles and its relationship to D-antigenicity and potency testing in rats.
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The use of a density gradient procedure for the quantification of intact, inactivated poliovirus particles in vaccine preparations is described. The procedure is both sensitive and highly reproducible and the results correlate with those of potency tests in rats and with D-antigen content as measured by ELISA. Because of the occasional ambiguity observed with D-antigen assays, it is suggested that the density gradient procedure will provide valuable additional information for the in vitro assessment of inactivated poliovirus preparations.
- Enzyme-Linked Immunosorbent Assay
- Antigens, Viral/*immunology
- 0 (Antigens, Viral)
- 0 (Poliovirus Vaccine, Inactivated)
- Centrifugation, Density Gradient/methods
- Poliovirus Vaccine, Inactivated/*analysis/immunology/pharmacology