Induction of neutralizing antibody in mice against poliovirus type II with monoclonal anti-idiotypic antibody.
January 1985
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Syngeneic monoclonal anti-idiotope antibody Ab2,2-17C3SCC was raised against an idiotope on a protective monoclonal antibody with specificity for poliovirus type II. Ab2,2-17C3SCC detects a paratope-related interspecies IdX. Ab2,2-17C3SCC purified from supernatant fluids of hybridoma cells by protein A-Sepharose was injected into 4- to 6-wk-old BALB/c mice. The sera of the mice were screened for the expression of antibodies bearing the corresponding idiotope. Immunization of mice with Ab2,2-17C3SCC induced antibodies of complementary specificity. Furthermore, micro VN tests suggest that Ab2,2-17C3SCC can substitute for antigen in the induction of anti-polio neutralizing antibodies, and hence can function as a monoclonal anti-idiotypic vaccine.
- Animals
- Mice
- Antibodies, Viral/*biosynthesis
- Mice, Inbred BALB C
- Binding, Competitive
- Antigens, Viral/immunology
- 0 (Antibodies, Monoclonal)
- Immunization, Passive
- Neutralization Tests
- Cross Reactions
- 0 (Antigens, Viral)
- 0 (Antibodies, Viral)
- Polioviruses/*immunology
- 0 (Immunoglobulin Idiotypes)
- Antibodies, Monoclonal/*administration & dosage/analysis/biosynthesis
- Antigen-Antibody Reactions
- Immunoglobulin Idiotypes/*immunology
- antibody
- polioviru
- idiotope
- 2.2-17c
- plate
- balb /c mice
- binding
- protein
- neutralizing
- elisa
- inhibition
- control
- anti-polioviru
- ab 2.2-17c
- strain
- antigen
- response
- anti-idiotypic
- 1-10 c 9e
- vaccine
