Comparison of an enzyme-linked immunosorbent assay, an immunofluorescence assay and a hemagglutination inhibition assay for detection of antibodies to K-papovavirus in mice.
View PDF Version
The sensitivity of a newly developed enzyme-linked immunosorbent assay (ELISA) for detection of antibody to K virus was compared with the sensitivities of an immunofluorescence assay (IFA) and a hemagglutination inhibition assay (HIA). Specific pathogen-free BALB/c RIVM mice, 5 weeks old, were inoculated intraperitoneally with a mouse organ suspension containing 10(4.5) TCID50 of K virus per dose. Control animals were inoculated with a control mouse organ suspension. No clinical signs were observed during the 7 weeks they were followed for the development of serum antibody. The ELISA proved to be the most sensitive of the three assays and demonstrated K virus-specific antibodies as early as 3 days after infection.
- Comparative Study
- Predictive Value of Tests
- Specific Pathogen-Free Organisms
- Enzyme-Linked Immunosorbent Assay
- Mice, Inbred BALB C
- Hemagglutination Inhibition Tests
- Fluorescent Antibody Technique
- 0 (Antibodies, Viral)
- Antibodies, Viral/*analysis
- Tumor Virus Infections/*immunology