Induction and characterization of monoclonal anti-idiotypic antibodies reactive with idiotopes of canine parvovirus neutralizing monoclonal antibodies.
January 1991
Article
| Related Files |
|---|
View PDF Version
(eur_osterhaus_9109.pdf, 1.0MB) |
|
Redirect to publisher's version
(publisher's version.url.txt, 48 bytes) |
Monoclonal anti-idiotypic (anti-Id) antibodies (Ab2) were generated against idiotypes (Id) of canine parvovirus (CPV) specific monoclonal antibodies (MoAbs). The binding of most of these anti-Id antibodies to their corresponding Id could be inhibited by antigen, thus classifying these anti-Id antibodies as Ab2 gamma or Ab2 beta. By inhibiting experiments it was shown that these anti-Id antibodies did not recognize interspecies cross-reactive idiotopes, but recognized private idiotopes, uniquely associated with the Id of the anti-CPV MoAb used for immunization. This classifies these anti-Id antibodies as non-internal image Ab2 gamma. The potential use of these non-internal image anti-Id antibodies for the induction of antiviral antibodies in the CPV system is discussed.
- Cats
- Animals
- Mice
- Dogs
- Enzyme-Linked Immunosorbent Assay
- Mice, Inbred BALB C
- Species Specificity
- 0 (Antibodies, Monoclonal)
- 0 (Epitopes)
- 0 (Immunoglobulin G)
- 0 (Immunoglobulin Idiotypes)
- 0 (Antibodies, Anti-Idiotypic)
- Immunoglobulin Idiotypes/immunology
- Parvoviridae/*immunology
- Antibodies, Anti-Idiotypic/*immunology
- Antibodies, Monoclonal/biosynthesis/*immunology
- Binding, Competitive/immunology
- Cross Reactions/immunology
- Epitopes/*immunology
- Immunoglobulin G/analysis
- Parvoviridae Infections/*veterinary
- Dog Diseases/*immunology
- antibody
- anti-id
- anti-id antibodies
- elisa
- idiotope
- anti-id moabs
- inhibition
- binding
- virus
- plate
- response
- antigen
- anti-cpv
- anti-ld
- balb /c mice
- parvoviru
- image
- fragment
- neutralizing
- mouse
