Measles virus transmembrane fusion protein synthesized de novo or presented in iscom is endogenously processed for HLA class I- and class II-restricted cytotoxic T cell recognition.
January 1992
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The routes used by antigen-presenting cells (APC) to convert the transmembrane fusion glycoprotein (F) of measles virus (MV) to HLA class I and class II presentable peptides have been examined, using cloned cytotoxic T lymphocytes in functional assays. Presentation by Epstein-Barr virus-transformed B lymphoblastoid cell lines was achieved using live virus, ultraviolet light-inactivated virus, and purified MV-F delivered either as such or incorporated in immunostimulating complexes (MV-F-ISCOM). Only live virus and MV-F-ISCOM allow presentation by class I molecules, while all antigen preparations permit class II-restricted presentation. We observe presentation of MV-F from live virus and as MV-F-ISCOM by class II molecules in a fashion that is not perturbed by chloroquine. Our studies visualize novel presentation pathways of type I transmembrane proteins.
- Animals
- Human
- Support, Non-U.S. Gov't
- T-Lymphocytes, Cytotoxic/*immunology
- 0 (Viral Fusion Proteins)
- Measles virus/*immunology
- *Lymphocyte Activation
- 0 (Antigens, CD4)
- 0 (Antigens, CD8)
- Antigens, CD4/analysis
- 0 (Histocompatibility Antigens Class I)
- Antigen-Presenting Cells/physiology
- Antigens, CD8/analysis
- Histocompatibility Antigens Class I/*immunology
- Histocompatibility Antigens Class II/*immunology
- Viral Fusion Proteins/*biosynthesis/immunology
- 0 (Histocompatibility Antigens Class II)
- class
- peptide
- class i
- protein
- antigen
- clone
- b-lcl
- presentation
- mv-f-iscom
- class ii molecules
- molecule
- virus
- iscom
- processing
- ii-restricted
- class i molecules
- pathway
- nature
- target
- 1990.
