Construction and characterisation of infectious recombinant HIV-1 clones containing CTL epitopes from structural proteins in Nef.
January 2002
Article
volume 99, issue 1-2 pp 115-121.
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In this study the construction is described of HIV-1 molecular clones in which CTL epitopes from RT or Env late proteins were inserted into the Nef early protein. The ectopic epitopes were efficiently processed from the recombinant Nef proteins, were recognized by their cognate CTL in cytolytic assays, and did not perturb virus replication or viral protein expression in vitro. These recombinant viruses will therefore be an important tool in studying the effect of distinct epitope expression kinetics on the efficiency of CTL-mediated suppression of HIV-1 replication.
Keywords
- Cell Line
- Humans
- Amino Acid Sequence
- T-Lymphocytes, Cytotoxic/*immunology
- Virus Replication
- *Recombination, Genetic
- Cloning, Molecular/methods
- Epitopes, T-Lymphocyte/*immunology
- Gene Products, nef/*genetics/immunology
- Gene Products, env/genetics/immunology
- HIV Reverse Transcriptase/genetics/immunology
- HIV-1/genetics/*pathogenicity/physiology
- nef Gene Products, Human Immunodeficiency Virus
Automatically Extracted Terms
- epitope
- virus
- protein
- expression
- van baalen
- replication
- ctl epitopes
- clone
- nef gene
- ectopic epitopes
- guillon
- virol
- tcl 2h cells
- / journal
- baalen
- immunodeficiency
- kinetic
- insertion
- cytotoxic t lymphocytes
- antibodies eva 3068.1
