Decreased DNA repair capacity in familial, but not in sporadic Alzheimer's disease
January 1991
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Using the alkaline filter elution technique we determined the induction and disappearance of DNA single-strand breaks (SSB) in freshly isolated peripheral blood lymphocytes (PBL) from 43 Alzheimer's disease (AD) patients and 48 normal, healthy age- and sex-matched control subjects following in vitro exposure to N-ethyl-N-nitrosourea (ENU). The mean percentage SSB disappearance in PBL from control subjects at 1 h after ENU treatment was 41.4 +/- 2.9%; this was not significantly different from that found in samples from AD patients which had no (n = 16) or one (n = 12) first-degree relative with dementia (42.5 +/- 8.2% and 43.0 +/- 4.4%, respectively; p greater than 0.75). However, in PBL of 15 AD patients with at least two first-degree relatives with dementia the mean percentage SSB disappearance was 23.6 +/- 5.8%, which was significantly lower than that found in controls (p less than 0.01) or in the other AD patients (p less than 0.02).
- Male
- Human
- Support, Non-U.S. Gov't
- Aged
- Female
- Middle Aged
- DNA Damage
- dementia
- Aging/metabolism
- 759-73-9 (Ethylnitrosourea)
- Alzheimer Disease/genetics/metabolism
- DNA Repair/physiology
- Ethylnitrosourea/diagnostic use
- Lymphocytes/metabolism/physiology
- patient
- ad patients
- repair
- control
- disease
- percentage ssb disappearance
- subject
- elution
- alzheimer
- disappearance
- dementia
- dna repair
- control subjects
- percentage
- group
- study
- ad patient
- rpmi 1640 medium
- 48 control subjects
- 2 mm glutamine
