Genetic characterization of the unique short segment of phocid herpesvirus type 1 reveals close relationships among alphaherpesviruses of hosts of the order Carnivora
January 2003
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To further characterize phocid herpesvirus type 1 (PhHV-1) at the molecular level, a cluster of genes comprising the complete unique short (Us) region of PhHV-1 has been cloned and sequenced. Within this region, ORFs were detected that code for the equivalent of the Us 2- protein of herpes simplex virus (HSV), a putative protein kinase, and for the glycoprotein equivalents gG, gD, gI and gE. In addition, two small ORFs downstream of gE, homologous to the Us 8.5 and Us 9 proteins of HSV were identified. Comparative analysis of the ORF encoding the gD equivalent of PhHV-1 identified the corresponding proteins of the alphaherpesviruses canine herpesvirus and, to lesser degree, feline herpesvirus as the closest relatives.
Keywords
- Animals
- Comparative Study
- Cloning, Molecular
- Phylogeny
- Species Specificity
- Genes, Viral
- Viral Proteins/genetics
- Multigene Family
- Open Reading Frames
- Alphaherpesvirinae/classification/*genetics/isolation & purification
- Carnivora/virology
- Seals, Earless/*virology
Automatically Extracted Terms
- herpesviru
- protein
- osterhau
- sequence
- region
- equivalent
- virus
- phocid
- fragment
- probe
- alphaherpesviruse
- transcript
- phylogenetic
- homology
- characterization
- virology
- segment
- psort ii software
- orfs encoding gg
- n-linked glycosylation sites
