Expression of haemopexin receptors by cultured human cytotrophoblast
January 1995
Article
| Related Files |
|---|
View PDF Version
(7741695.pdf, 0.6MB) |
The expression of cell-surface haemopexin (Hx) receptors on human cytotrophoblasts was assessed by using four different Hx species purified from plasma: human Hx isolated by wheatgerm-affinity chromatography, human Hx isolated by haem-agarose-affinity chromatography and rabbit and rat Hx, also isolated by haem-agarose-affinity chromatography. About 3500-7000 high-affinity (Kd 0.34-0.85 nM) receptors per cell were measured by Scatchard-type analysis at 4 degrees C using human (species obtained by both methods) or rabbit 125I-labelled haem-Hx. Measured simultaneously, transferrin receptor number and affinity were 40,000/cell and 0.83 nM respectively. In contrast with transferrin receptors, the number of Hx receptors did not increase during 24 h in cytotrophoblast culture. Rat Hx showed no specific binding to human Hx receptors in cytotrophoblast cultures.
- Animals
- Comparative Study
- Female
- Humans
- Research Support, Non-U.S. Gov't
- Research Support, U.S. Gov't, P.H.S.
- Rats
- Cells, Cultured
- Pregnancy
- Kinetics
- Species Specificity
- Rabbits
- Hemopexin/metabolism
- Receptors, Peptide/metabolism/*physiology
- Receptors, Transferrin/metabolism
- Trophoblasts/*metabolism/*ultrastructure
- binding
- receptor
- culture
- tf -2fe
- medium
- haem-hx
- number
- cytotrophoblast
- experiment
- rat hx
- method
- expression
- rabbit
- non-specific binding
- hx receptors
- non-specific
- van dijk
- tfr expression
- species
- result
