Tissue specific and androgen-regulated expression of human prostate-specific transglutaminase
January 1996
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Transglutaminases (TGases) are calcium-dependent enzymes catalysing the post-translational cross-linking of proteins. In the prostate at least two TGases are present, the ubiquitously expressed tissue-type TGase (TGC), and a prostate-restricted TGase (TGP). This paper deals with the molecular cloning and characterization of the cDNA encoding the human prostate TGase (hTGP). For this purpose we have screened a human prostate cDNA library with a probe from the active-site region of TGC. The largest isolated cDNA contained an open reading frame encoding a protein of 684 amino acids with a predicted molecular mass of 77 kDa as confirmed by in vitro transcription-translation and subsequent SDS/PAGE. The hTGP gene was tissue-specifically expressed in the prostate, yielding an mRNA of approx. 3.5 kb. Furthermore, a 3-fold androgen-induced upregulation of hTGP mRNA expression has been demonstrated in the recently developed human prostate cancer cell line, PC346C. Other well established human prostate cancer cell lines, LNCaP and PC-3, showed no detectable hTGP mRNA expression on a Northern bolt. The gene coding for prostate TGase was assigned to chromosome 3.
- Male
- Animals
- Female
- Humans
- Amino Acid Sequence
- Cloning, Molecular
- Mice
- Molecular Sequence Data
- Base Sequence
- Tumor Cells, Cultured
- Cricetinae
- Sequence Homology, Amino Acid
- Chromosome Mapping
- Hybrid Cells
- Tissue Distribution
- DNA, Complementary/genetics
- Gene Expression Regulation, Enzymologic
- Androgens/*metabolism
- DNA Probes/genetics
- Chromosomes, Human, Pair 3/genetics
- Prostate/*enzymology
- Prostatic Neoplasms/enzymology/genetics
- Transglutaminases/genetics/*metabolism
- tgase
- prostate
- clone
- sequence
- protein
- expression
- htgp cdna
- figure
- prostate cdna library
- cancer
- result
- presence
- htgp mrna
- chromosome
- acid sequence
- region
- product
- pcdna
- pc 346c cells
- fragment
