Enzymatic method for inactivation of aminoglycosides during measurement of postantibiotic effect
January 1996
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To determine the postantibiotic effect of aminoglycosides, two methods are currently being used to remove the test drug: repeated washing and dilution. An enzymatic inactivation method of removing gentamicin and tobramycin was developed and compared with the dilution method. This enzymatic method provides a rapid and simple alternative method of removing aminoglycosides which results in reliable postantibiotic-effect values.
Keywords
- Comparative Study
- Acetyltransferases/*pharmacology
- Bacteria/drug effects/*metabolism
- Gentamicins/metabolism
- Microbial Sensitivity Tests/*methods
- Tobramycin/metabolism
- Anti-Bacterial Agents/*metabolism
Automatically Extracted Terms
- method
- tobramycin
- culture
- inactivation
- dilution
- 24- h culture
- inactivation method
- enzyme
- aminoglycoside
- aureus
- dilution method
- antibiotic
- acetyl coenzyme
- gentamicin
- solution
- pae values
- value
- effect
- activity
- 0.5 mg /l
