Comprehensive study of several general and type-specific primer pairs for detection of human papillomavirus DNA by PCR in paraffin-embedded cervical carcinomas
January 1996
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We have compared the efficacies of three general primer pairs for the detection of human papillomavirus (HPV) DNA in formaldehyde-fixed paraffin-embedded carcinomas. The use of these primer pairs leads to underestimates of the HPV prevalence (GP5/6, 61.1%; CPI/IIG, 57.4%; MY09/11, 46.9%; combined, 72.8%). The efficacy of each primer pair seemed to be inversely correlated to the length of the amplimer produced. By using newly developed type-specific primer pairs (amplimer length, approximately 100 bp), an increase in HPV DNA detection (87.6%) was found.
Keywords
- Female
- Humans
- Research Support, Non-U.S. Gov't
- Molecular Sequence Data
- Base Sequence
- DNA, Viral/*analysis
- *Polymerase Chain Reaction
- *DNA Primers
- Papillomavirus, Human/*genetics
- Uterine Cervical Neoplasms/*virology
Automatically Extracted Terms
- primer
- primers
- carcinoma
- polymerase chain reaction
- primer pairs
- detection
- ts primers
- ts primer pairs
- hpv dna
- amplification
- polymerase
- reaction
- chain
- tissue
- study
- probe
- cancer
- sample
- papillomaviru
- amplimer
