Cleavage at a V(D)J recombination signal requires only RAG1 and RAG2 proteins and occurs in two steps
1995-11-03
Article
volume 83, issue 3 pp 387-395.
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Formation of double-strand breaks at recombination signal sequences is an early step in V(D)J recombination. Here we show that purified RAG1 and RAG2 proteins are sufficient to carry out this reaction. The cleavage reaction can be divided into two distinct steps. First, a nick is introduced at the 5' end of the signal sequence. The other strand is then broken, resulting in a hairpin structure at the coding end and a blunt, 5'-phosphorylated signal end. The hairpin is made as a direct consequence of the cleavage mechanism. Nicking and hairpin formation each require the presence of a signal sequence and both RAG proteins.
Keywords
- Nucleic Acid Conformation
- Amino Acid Sequence
- Molecular Sequence Data
- Base Sequence
- humans
- DNA/metabolism
- Nuclear Proteins
- *DNA-Binding Proteins
- Recombination, Genetic
- *Homeodomain Proteins
- Genes, RAG-1/*physiology
- Hela Cells/physiology
- Protein Sorting Signals/genetics
- Proteins/*genetics/isolation & purification/metabolism
Automatically Extracted Terms
- protein
- signal
- rag 1
- cleavage
- rag 2 proteins
- hairpin
- recombination
- product
- substrate
- reaction
- coding
- rag 2
- sequence
- strand
- figure
- oligonucleotide
- formation
- rag proteins
- phosphodiester bond
- expression
