Development of eosinophilic inflammation is independent of B–T cell interaction in a chronic house dust mite-driven asthma model
Clinical and Experimental Allergy , Volume 47 - Issue 4 p. 551- 564
Background: Chronic exposure to environmental triggers, such as house dust mite (HDM), drives T helper 2 (Th2) cell-mediated asthma. Recent evidence has shown that B–T cell interaction, and in particular germinal centre reactions and follicular T helper (Tfh) cells are required for the development of eosinophilic airway inflammation in HDM-driven models containing a sensitization and challenge phase. Whether B–T cell interactions are essential for pulmonary eosinophilic inflammation following chronic allergen provocation remains unknown. Aims: In this study, we investigated the importance of B–T cell interaction in the development of eosinophilic airway inflammation and pulmonary remodelling in a chronic HDM-driven asthma model. Methods: We exposed C57BL/6, Cd40l−/−, and Mb1−/− mice to HDM three times a week for five consecutive weeks. Results: Chronic HDM exposure induced a pronounced eosinophilic allergic airway inflammation in broncho-alveolar lavage fluid (BALf) and lung tissue, associated with the formation of immunologically active inducible bronchus-associated lymphoid tissue (iBALT) in the lungs. The absence of B cells or lack of CD40L signalling did not hamper eosinophilic inflammation in the airways, although the number of Tfh and Th2 cells was substantially reduced in the lungs. Importantly, type 2 innate lymphoid cell (ILC2) numbers in BALf and lung were not affected by the absence of B cells or B–T cell interaction. Furthermore, eosinophilic airway inflammation is not sufficient to induce pulmonary remodelling and airway hyperresponsiveness. Conclusion and Clinical Relevance: From these findings, we conclude that B–T cell interaction is required for robust Tfh and Th2 cell induction, but not essential for eosinophilic airway inflammation during a chronic HDM-driven asthma model.
|, , , ,
|Clinical and Experimental Allergy
|Department of Pulmonology
Vroman, H., Bergen, I., Li, B., van Hulst, J., Lukkes, M., van Uden, D., … Kool, M. (2017). Development of eosinophilic inflammation is independent of B–T cell interaction in a chronic house dust mite-driven asthma model. Clinical and Experimental Allergy, 47(4), 551–564. doi:10.1111/cea.12834