2017-08-01
Analysis of SHIP1 expression and activity in Crohn’s disease patients
Publication
Publication
PLoS ONE , Volume 12 - Issue 8
Background
SH2 domain containing inositol-5-phosphatase (SHIP1) is an important modulator of innate
and adaptive immunity. In mice, loss of SHIP1 provokes severe ileitis resembling Crohn's
disease (CD), as a result of deregulated immune responses, altered cytokine production
and intestinal fibrosis. Recently, SHIP1 activity was shown to be correlated to the presence
of a CD-associated single nucleotide polymorphism in ATG16L1. Here, we studied SHIP1
activity and expression in an adult cohort of CD patients.
Methods
SHIP1 activity, protein and mRNA expression in peripheral blood mononuclear cells from
CD patients in clinical remission were determined by Malachite green assay, Western blotting
and qRT-PCR respectively. Genomic DNA was genotyped for ATG16L1 rs2241880.
Results
SHIP1 protein levels are profoundly diminished in a subset of patients; however, SHIP1
activity and expression are not correlated to ATG16L1 SNP status in this adult cohort.
Conclusions
Aberrant SHIP1 activity can contribute to disease in a subset of adult CD patients, and warrants
further investigation.
| Additional Metadata | |
|---|---|
| doi.org/10.1371/journal.pone.0182308, hdl.handle.net/1765/100939 | |
| PLoS ONE | |
| Organisation | Department of Gastroenterology & Hepatology |
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Somasundaram, R., Fernandes, S., Deuring, J., de Haar, C., Kuipers, E., Vogelaar, L., … Fuhler, G. (2017). Analysis of SHIP1 expression and activity in Crohn’s disease patients. PLoS ONE, 12(8). doi:10.1371/journal.pone.0182308 |
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