Interleukin-17 receptor A (IL-17RA) as a central regulator of the protective immune response against Giardia
The protozoan parasite Giardia is a highly prevalent intestinal pathogen with a wide host range. Data obtained in mice, cattle and humans revealed the importance of IL-17A in the development of a protective immune response against Giardia. The aim of this study was to further unravel the protective effector mechanisms triggered by IL-17A following G. muris infection in mice, by an RNA-sequencing approach. C57BL/6 WT and C57BL/6 IL-17RA KO mice were orally infected with G. muris cysts. Three weeks post infection, intestinal tissue samples were collected for RNA-sequencing, with samples from uninfected C57BL/6 WT and C57BL/6 IL-17RA KO animals serving as negative controls. Differential expression analysis showed that G. muris infection evoked the transcriptional upregulation of a wide array of genes, mainly in animals with competent IL-17RA signaling. IL-17RA signaling induced the production of various antimicrobial peptides, such as angiogenin 4 and α- and β-defensins and regulated complement activation through mannose-binding lectin 2. The expression of the receptor that regulates the secretion of IgA into the intestinal lumen, the polymeric immunoglobulin receptor, was also dependent on IL-17RA signaling. Interestingly, the transcriptome data showed for the first time the involvement of the circadian clock in the host response following Giardia infection.
|Persistent URL||dx.doi.org/10.1038/s41598-017-08590-x, hdl.handle.net/1765/101559|
Paerewijck, O. (Oonagh), Maertens, B. (Brecht), Dreesen, L, Van Meulder, F. (Frederik), Peelaers, I. (Iris), Ratman, D. (Dariusz), … Geldhof, P. (2017). Interleukin-17 receptor A (IL-17RA) as a central regulator of the protective immune response against Giardia. Scientific Reports, 7(1). doi:10.1038/s41598-017-08590-x