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W.B. van Leeuwen (Willem), C. Jay (Corinne), S.V. Snijders (Susan), N. Durin (Nathalia), B. Lacroix (Bruno), H.A. Verbrugh (Henri), M.C. Enright (Mark), A. Troesch (Alain) and A.F. van Belkum (Alex)

2003-07-01

Multilocus sequence typing of Staphylococcus aureus with DNA array technology

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Journal of Clinical Microbiology , Volume 41 - Issue 7 p. 3323- 3326

A newly developed oligonucleotide array suited for multilocus sequence typing (MLST) of Staphylococcus aureus strains was analyzed with two strain collections in a two-center study. MLST allele identification for the first strain collection fully agreed with conventional strain typing. Analysis of strains from the second collection revealed that chip-defined MLST was concordant with conventional MLST. Array-mediated MLST data were reproducible, exchangeable, and epidemiologically concordant.

Additional Metadata
Keywords *Sequence Analysis, DNA, Alleles, Bacterial Proteins/*genetics, Bacterial Typing Techniques, Humans, Methicillin Resistance, Oligonucleotide Array Sequence Analysis/*methods, Staphylococcal Infections/microbiology, Staphylococcus aureus/*classification/drug effects/*genetics
Persistent URL doi.org/10.1128/JCM.41.7.3323-3326.2003, hdl.handle.net/1765/10190
Series Staphylococcus aureus: Resources
Journal Journal of Clinical Microbiology
Organisation Erasmus MC: University Medical Center Rotterdam
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