A newly developed oligonucleotide array suited for multilocus sequence typing (MLST) of Staphylococcus aureus strains was analyzed with two strain collections in a two-center study. MLST allele identification for the first strain collection fully agreed with conventional strain typing. Analysis of strains from the second collection revealed that chip-defined MLST was concordant with conventional MLST. Array-mediated MLST data were reproducible, exchangeable, and epidemiologically concordant.

*Sequence Analysis, DNA, Alleles, Bacterial Proteins/*genetics, Bacterial Typing Techniques, Humans, Methicillin Resistance, Oligonucleotide Array Sequence Analysis/*methods, Staphylococcal Infections/microbiology, Staphylococcus aureus/*classification/drug effects/*genetics
dx.doi.org/10.1128/JCM.41.7.3323-3326.2003, hdl.handle.net/1765/10190
Staphylococcus aureus: Resources
Journal of Clinical Microbiology
Erasmus MC: University Medical Center Rotterdam

van Leeuwen, W.B, Jay, C, Snijders, S.V, Durin, N, Lacroix, B, Verbrugh, H.A, … van Belkum, A.F. (2003). Multilocus sequence typing of Staphylococcus aureus with DNA array technology. Journal of Clinical Microbiology, 41(7), 3323–3326. doi:10.1128/JCM.41.7.3323-3326.2003