2017-12-01
Genetic and epigenetic features direct differential efficiency of Xist-mediated silencing at X-chromosomal and autosomal locations
Publication
Publication
Nature Communications , Volume 8 - Issue 1
Xist is indispensable for X chromosome inactivation. However, how Xist RNA directs chromosome-wide silencing and why some regions are more efficiently silenced than others remains unknown. Here, we explore the function of Xist by inducing ectopic Xist expression from multiple different X-linked and autosomal loci in mouse aneuploid and female diploid embryonic stem cells in which Xist-mediated silencing does not lead to lethal functional monosomy. We show that ectopic Xist expression faithfully recapitulates endogenous X chromosome inactivation from any location on the X chromosome, whereas long-range silencing of autosomal genes is less efficient. Long interspersed elements facilitate inactivation of genes located far away from the Xist transcription locus, and genes escaping X chromosome inactivation show enrichment of CTCF on X chromosomal but not autosomal loci. Our findings highlight important genomic and epigenetic features acquired during sex chromosome evolution to facilitate an efficient X chromosome inactivation process.
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| doi.org/10.1038/s41467-017-00528-1, hdl.handle.net/1765/102144 | |
| Nature Communications | |
| Organisation | Department of Developmental Biology |
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Loda, A., Brandsma, J., Vassilev, I. (Ivaylo), Servant, N., Loos, F., Amirnasr, A., … Gribnau, J. (2017). Genetic and epigenetic features direct differential efficiency of Xist-mediated silencing at X-chromosomal and autosomal locations. Nature Communications, 8(1). doi:10.1038/s41467-017-00528-1 |
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