Zika virus (ZIKV) infections are a significant public health concern. A strong capability for ZIKV detection is an absolute requirement for adequate preparedness and response strategies and individual patient care. The objective of this study was to assess and improve the capability of European expert laboratories for molecular testing for ZIKV through an external quality assessment (EQA) scheme. Laboratories were provided a panel of 12 samples which included negative samples, samples containing African- or Asian-lineage ZIKV at various concentrations (103 to 109 copies/ml), and samples containing dengue virus, yellow fever virus, or chikungunya virus. The results were analyzed on the basis of the outcomes of testing for the samples and the extraction and detection method used. Samples with a ZIKV RNA status scored correctly by.

Additional Metadata
Keywords EQA, EVD-LabNet, External quality assessment, Laboratory preparedness, Laboratory response, Zika virus
Persistent URL dx.doi.org/10.1128/JCM.00987-17, hdl.handle.net/1765/102622
Journal Journal of Clinical Microbiology
Citation
Charrel, R, Mögling, R, Pas, S.D, Papa, A, Baronti, C. (Cecile), Koopmans, M.P.G, D.V.M., … Reusken, C.B.E.M. (2017). Variable sensitivity in molecular detection of zika virus in european expert laboratories: External quality assessment, November 2016. Journal of Clinical Microbiology, 55(11), 3219–3226. doi:10.1128/JCM.00987-17