Preferential HLA usage in the influenza virus-specific CTL response
To study whether individual HLA class I alleles are used preferentially or equally in human virus-specific CTL responses, the contribution of individual HLA-A and -B alleles to the human influenza virus-specific CTL response was investigated. To this end, PBMC were obtained from three groups of HLA-A and -B identical blood donors and stimulated with influenza virus. In the virus-specific CD8(+) T cell population, the proportion of IFN-gamma- and TNF-alpha-producing cells, restricted by individual HLA-A and -B alleles, was determined using virus-infected C1R cells expressing a single HLA-A or -B allele for restimulation of these cells. In HLA-B*2705- and HLA-B*3501-positive individuals, these alleles were preferentially used in the influenza A virus-specific CTL response, while the contribution of HLA-B*0801 and HLA-A*0101 was minor in these donors. The magnitude of the HLA-B*0801-restricted response was even lower in the presence of HLA-B*2705. C1R cells expressing HLA-B*2705, HLA-A*0101, or HLA-A*0201 were preferentially lysed by virus-specific CD8(+) T cells. In contrast, the CTL response to influenza B virus was mainly directed toward HLA-B*0801-restricted epitopes. Thus, the preferential use of HLA alleles depended on the virus studied.
|*Cytotoxicity Tests, Immunologic/statistics & numerical data, Adult, Alleles, Cell Line, Transformed, Comparative Study, Epitopes, T-Lymphocyte/immunology, HLA-A Antigens/biosynthesis/*genetics/immunology, HLA-B Antigens/biosynthesis/*genetics/immunology, HLA-B35 Antigen/genetics, Humans, Influenza A virus/*immunology, Influenza B virus/*immunology, Interferon Type II/biosynthesis, Middle Aged, Research Support, Non-U.S. Gov't, T-Lymphocytes, Cytotoxic/*immunology/metabolism/*virology, Transfection, Tumor Necrosis Factor-alpha/biosynthesis|
|Journal of Immunology|
|Organisation||Erasmus MC: University Medical Center Rotterdam|
Boon, A.C.M, de Mutsert, G, Fouchier, R.A.M, Sintnicolaas, K, Rimmelzwaan, G.F, & Osterhaus, A.D.M.E. (2004). Preferential HLA usage in the influenza virus-specific CTL response. Journal of Immunology. Retrieved from http://hdl.handle.net/1765/10325