We report here that the expression of the transcription factor T-bet, which is known to be required for effector cytotoxic CD8+ T lymphocytes (CTL) generation and effector memory cell formation, is regulated in CTL by microRNA-155 (miR-155). Importantly, we show that the proliferative effect of miR-155 on CD8+ T cells is mediated by T-bet. T-bet levels in CTL were controlled in vivo by miR-155 via SH2 (Src homology 2)-containing inositol phosphatase-1 (SHIP-1), a known direct target of miR-155, and SHIP-1 directly downregulated T-bet. Our studies reveal an important and unexpected signaling axis between miR-155, T-bet, and SHIP-1 in in vivo CTL responses and suggest an important signaling module that regulates effector CTL immunity.

Additional Metadata
Keywords CD8+ T cells, Influenza, MicroRNAs, MiR-155, Src homology 2-containing inositol phosphatase-1, T-bet
Persistent URL dx.doi.org/10.3389/fimmu.2017.01696, hdl.handle.net/1765/103438
Journal Frontiers in Immunology
Citation
Hope, J.L, Stairiker, C.J, Spantidea, P.I. (Panagiota I.), Gracias, D.T, Carey, A.J. (Alison J.), Fike, A.J, … Katsikis, P.D. (2017). The transcription factor T-Bet is regulated by MicroRNA-155 in murine anti-viral CD8+ T cells via SHIP-1. Frontiers in Immunology, 8(DEC). doi:10.3389/fimmu.2017.01696