Purpose: To analyze intraocular cytokine levels and cell profiles in patients with rubella virus-associated uveitis (RVU). Methods: We collected intraocular fluid samples from patients with RVU (n = 10), uveitis of other causes (n = 27), and cataract (n = 22). Levels of 15 cytokines (IL-1β, IL-1ra, IL-2, IL-6, IL-6rα, IL-7, IL-8, IL-10, IL-17A, IL-23, TARC, MCP-1, TNF-α, PlGF, and VEGF) were measured using multiplex assay, and intraocular cell populations were determined by multiparameter flowcytometry. Clinical characteristics of RVU patients were collected and compared to laboratory outcomes. Results: RVU patients exhibited high intraocular levels of MCP-1, IL-6rα, and TARC, whilst patients with noninfectious uveitis were characterized by high levels of PlGF. Cataract patients showed high levels of IL-2 and IL-23. Intraocular cell population of RVU patients disclosed mainly T-cells and monocytes/macrophages and B-cells were scarcely detected. Conclusion: RVU patients exhibit a cytokine profile distinct from noninfectious uveitis and cataract.

Cytokines, Fuchs uveitis syndrome, immunopathology, intraocular fluid, rubella-virus associated uveitis
dx.doi.org/10.1080/09273948.2017.1421671, hdl.handle.net/1765/104353
Ocular Immunology and Inflammation
Department of Ophthalmology

Fazil, Z. (Z.), ten Berge, J.C.E.M, Langerak, A.W, Rothová, A, & Dik, W.A. (2018). An Intraocular Inflammatory Profile of Rubella Associated Uveitis. Ocular Immunology and Inflammation, 1–6. doi:10.1080/09273948.2017.1421671