Idiopathic pulmonary fibrosis (IPF) is a fatal lung disease characterized by the progressive and irreversible destruction of lung architecture, which causes significant deterioration in lung function and subsequent death from respiratory failure. The pathogenesis of IPF in experimental animal models has been induced by bleomycin administration. In this study, we investigate an IPF-like mouse model induced by a double intratracheal bleomycin instillation. Standard histological assessments used for studying lung fibrosis are invasive terminal procedures. The goal of this work is to monitor lung fibrosis through noninvasive imaging techniques such as Fluorescent Molecular Tomography (FMT) and Micro-CT. These two technologies validated with histology findings could represent a revolutionary functional approach for real time non-invasive monitoring of IPF disease severity and progression. The fusion of different approaches represents a step further for understanding the IPF disease, where the molecular events occurring in a pathological condition can be observed with FMT and the subsequent anatomical changes can be monitored by Micro-CT.

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Keywords Animal models, Bleomycin, FMT, Idiopathic pulmonary fibrosis (IPF), Immunology and infection, In vivo imaging, Issue 134, Lung disease, Matrix metalloproteinases (MMPs), Micro-CT
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Journal Journal of Visualized Experiments
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Ruscitti, F. (Francesca), Ravanetti, F. (Francesca), Donofrio, G. (Gaetano), Ridwan, Y, van Heijningen, P.M, Essers, J, … Stellari, F.F. (Franco Fabio). (2018). A multimodal imaging approach based on micro-CT and fluorescence molecular tomography for longitudinal assessment of bleomycin-induced lung fibrosis in mice. Journal of Visualized Experiments, 2018(134). doi:10.3791/56443