Live-cell analysis of endogenous GFP-RPB1 uncovers rapid turnover of initiating and promoter-paused RNA Polymerase II
Proceedings of the National Academy of Sciences of the United States of America , Volume 115 - Issue 19 p. E4368- E4376
Initiation and promoter-proximal pausing are key regulatory steps of RNA Polymerase II (Pol II) transcription. To study the in vivo dynamics of endogenous Pol II during these steps, we generated fully functional GFP-RPB1 knockin cells. GFP-RPB1 photobleaching combined with computational modeling revealed four kinetically distinct Pol II fractions and showed that on average 7% of Pol II are freely diffusing, while 10% are chromatin-bound for 2.4 seconds during initiation, and 23% are promoter-paused for only 42 seconds. This unexpectedly high turnover of Pol II at promoters is most likely caused by premature termination of initiating and promoter-paused Pol II and is in sharp contrast to the 23 minutes that elongating Pol II resides on chromatin. Our live-cell–imaging approach provides insights into Pol II dynamics and suggests that the continuous release and reinitiation of promoter-bound Pol II is an important component of transcriptional regulation.
|Live-cell imaging, Promoter-proximal pausing, RNA Polymerase II, Transcription, Transcription dynamics|
|Proceedings of the National Academy of Sciences of the United States of America|
|Organisation||Erasmus MC: University Medical Center Rotterdam|
Steurer, B, Janssens, R, Geverts, B, Geijer, M.E. (Marit E.), Wienholz, F. (Franziska), Theil, A.F, … Marteijn, J.A. (2018). Live-cell analysis of endogenous GFP-RPB1 uncovers rapid turnover of initiating and promoter-paused RNA Polymerase II. Proceedings of the National Academy of Sciences of the United States of America, 115(19), E4368–E4376. doi:10.1073/pnas.1717920115