EB1 and EB3 regulate microtubule minus end organization and Golgi morphology
The Journal of Cell Biology , Volume 216 - Issue 10 p. 3179- 3198
End-binding proteins (EBs) are the core components of microtubule plus end tracking protein complexes, but it is currently unknown whether they are essential for mammalian microtubule organization. Here, by using CRI SPR/Cas9- mediated knockout technology, we generated stable cell lines lacking EB2 and EB3 and the C-terminal partner-binding half of EB1. These cell lines show only mild defects in cell division and microtubule polymerization. However, the length of CAM SAP2-decorated stretches at noncentrosomal microtubule minus ends in these cells is reduced, microtubules are detached from Golgi membranes, and the Golgi complex is more compact. Coorganization of microtubules and Golgi membranes depends on the EB1/EB3-myomegalin complex, which acts as membrane-microtubule tether and counteracts tight clustering of individual Golgi stacks. Disruption of EB1 and EB3 also perturbs cell migration, polarity, and the distribution of focal adhesions. EB1 and EB3 thus affect multiple interphase processes and have a major impact on microtubule minus end organization.
|The Journal of Cell Biology|
|This work was funded by the European Commission 7th Framework Programme; grant id erc/609822 - Building a Model Cell to Achieve Control of Cellular Organization (MODELCELL)|
|Organisation||Erasmus MC: University Medical Center Rotterdam|
Yang, C. (Chao), Wu, J, de Heus, C. (Cecilia), Grigoriev, I, Liv, N, Yao, Y, … Akhmanova, A.S. (2017). EB1 and EB3 regulate microtubule minus end organization and Golgi morphology. The Journal of Cell Biology, 216(10), 3179–3198. doi:10.1083/jcb.201701024