Dynamic Organization of Chromatin Domains Revealed by Super-Resolution Live-Cell Imaging
Molecular Cell , Volume 67 - Issue 2 p. 282- 293.e7
The eukaryotic genome is organized within cells as chromatin. For proper information output, higher-order chromatin structures can be regulated dynamically. How such structures form and behave in various cellular processes remains unclear. Here, by combining super-resolution imaging (photoactivated localization microscopy [PALM]) and single-nucleosome tracking, we developed a nuclear imaging system to visualize the higher-order structures along with their dynamics in live mammalian cells. We demonstrated that nucleosomes form compact domains with a peak diameter of ∼160 nm and move coherently in live cells. The heterochromatin-rich regions showed more domains and less movement. With cell differentiation, the domains became more apparent, with reduced dynamics. Furthermore, various perturbation experiments indicated that they are organized by a combination of factors, including cohesin and nucleosome-nucleosome interactions. Notably, we observed the domains during mitosis, suggesting that they act as building blocks of chromosomes and may serve as information units throughout the cell cycle.
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|Organisation||Erasmus MC: University Medical Center Rotterdam|
Nozaki, T. (Tadasu), Imai, R. (Ryosuke), Tanbo, M. (Mai), Nagashima, R. (Ryosuke), Tamura, S. (Sachiko), Tani, T. (Tomomi), … Maeshima, K. (Kazuhiro). (2017). Dynamic Organization of Chromatin Domains Revealed by Super-Resolution Live-Cell Imaging. Molecular Cell, 67(2), 282–293.e7. doi:10.1016/j.molcel.2017.06.018