An automated confocal Raman microspectrometer for rapid measurement of molecular concentration profiles in the skin is described. It permits the successive collection of Raman spectra at a range of depths below the skin surface. The axial resolution of the confocal Raman microspectrometer is 5.1 +/- 0.2 mu m. The setup was applied to determine water concentration profiles of the stratum corneum and to determine changes therein as a result of hydration of the skin.

, ,;2-7,
Journal of Raman Spectroscopy
Erasmus MC: University Medical Center Rotterdam

Caspers, P., Lucassen, G., Bruining, H., & Puppels, G. (2000). Automated depth-scanning confocal Raman microspectrometer for rapid in vivo determination of water concentration profiles in human skin. Journal of Raman Spectroscopy. doi:AID-JRS573%3E3.0.CO;2-7