Isolating Pediatric Mesenchymal Stem Cells with Enhanced Expansion and Differentiation Capabilities
Mesenchymal stem cells/marrow stromal cells (MSCs) are attractive for applications ranging from research and development to use in clinical therapeutics. However, the most commonly studied MSCs, adult bone marrow MSCs (A-MSCs), are limited by significant donor variation resulting in inconsistent expansion rates and multilineage differentiation capabilities. We have recently obtained permission to isolate pediatric MSCs (P-MSCs) from surplus iliac crest bone chips. Here, we developed a simple and easily replicable isolation protocol yielding P-MSCs, which adhere to MSC defining guidelines. After confirming immunophenotypic marker expression, we compared expansion rates, senescence, morphology, and trilineage differentiation of P-MSCs to A-MSCs for multiple donors. We found P-MSCs have faster in vitro replication, consistently show significantly lower senescence, and are capable of more reproducible multilineage differentiation than A-MSCs. We, therefore, believe P-MSCs are a promising candidate for use in research applications and potentially as part of an allogeneic therapeutic treatment.
|Keywords||differentiation, immunomodulation, marrow stromal cells, mesenchymal stem cells, MSC immunophenotype, pediatric mesenchymal stem cells, senescence|
|Persistent URL||dx.doi.org/10.1089/ten.tec.2018.0031, hdl.handle.net/1765/108894|
|Journal||Tissue Engineering - Part C: Methods|
Knuth, C.A, Kiernan, C.H. (Caoimhe H.), Palomares Cabeza, V. (Virginia), Lehmann, J, Witte-Bouma, J, ten Berge, D, … Farrell, E. (2018). Isolating Pediatric Mesenchymal Stem Cells with Enhanced Expansion and Differentiation Capabilities. Tissue Engineering - Part C: Methods, 24(6), 313–321. doi:10.1089/ten.tec.2018.0031