2018-06-01
Isolating Pediatric Mesenchymal Stem Cells with Enhanced Expansion and Differentiation Capabilities
Publication
Publication
Tissue Engineering - Part C: Methods , Volume 24 - Issue 6 p. 313- 321
Mesenchymal stem cells/marrow stromal cells (MSCs) are attractive for applications ranging from research and development to use in clinical therapeutics. However, the most commonly studied MSCs, adult bone marrow MSCs (A-MSCs), are limited by significant donor variation resulting in inconsistent expansion rates and multilineage differentiation capabilities. We have recently obtained permission to isolate pediatric MSCs (P-MSCs) from surplus iliac crest bone chips. Here, we developed a simple and easily replicable isolation protocol yielding P-MSCs, which adhere to MSC defining guidelines. After confirming immunophenotypic marker expression, we compared expansion rates, senescence, morphology, and trilineage differentiation of P-MSCs to A-MSCs for multiple donors. We found P-MSCs have faster in vitro replication, consistently show significantly lower senescence, and are capable of more reproducible multilineage differentiation than A-MSCs. We, therefore, believe P-MSCs are a promising candidate for use in research applications and potentially as part of an allogeneic therapeutic treatment.
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| , , , , , , | |
| doi.org/10.1089/ten.tec.2018.0031, hdl.handle.net/1765/108894 | |
| Tissue Engineering - Part C: Methods | |
| Organisation | Department of Oral and Maxillofacial Surgery |
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Knuth, C., Kiernan, C.H. (Caoimhe H.), Palomares Cabeza, V. (Virginia), Lehmann, J., Witte-Bouma, J., ten Berge, D., … Farrell, E. (2018). Isolating Pediatric Mesenchymal Stem Cells with Enhanced Expansion and Differentiation Capabilities. Tissue Engineering - Part C: Methods, 24(6), 313–321. doi:10.1089/ten.tec.2018.0031 |
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