Over the last decade, research on distinct types of CRISPR systems has revealed many structural and functional variations. Recently, several novel types of single-polypeptide CRISPR-associated systems have been discovered including Cas12a/Cpf1 and Cas13a/C2c2. Despite distant similarities to Cas9, these additional systems have unique structural and functional features, providing new opportunities for genome editing applications. Here, relevant fundamental features of natural and engineered CRISPR-Cas variants are compared. Moreover, practical matters are discussed that are essential for dedicated genome editing applications, including nuclease regulation and delivery, target specificity, as well as host repair diversity.

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Persistent URL dx.doi.org/10.1038/s41589-018-0080-x, hdl.handle.net/1765/108971
Journal Nature Chemical Biology
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Wu, W.Y. (Wen Y.), Lebbink, J.H.G, Kanaar, R, Geijsen, N, & van der Oost, J. (2018). Genome editing by natural and engineered CRISPR-associated nucleases. Nature Chemical Biology (Vol. 14, pp. 642–651). doi:10.1038/s41589-018-0080-x