The generation of allergen-specific TCR transgenic animals allows for the characterization of allergen-specific T-cell responses in vivo and in vitro and is a powerful tool to study adaptive immunity to allergens. Here we describe an approach starting from the isolation of antigen-specific T-cell hybridomas and using PCR, flow cytometric, and co-culture methods to obtain antigen-specific MHC class II-restricted CD4+ TCR transgenic mice on the Rag2−/− background.

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Keywords Allergen, Hybridoma, T-cell fusion, T-cell receptor, Transgenic
Persistent URL dx.doi.org/10.1007/978-1-4939-7896-0_15, hdl.handle.net/1765/109209
Series Methods in Molecular Biology
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Citation
van Heerswynghels, M, Toussaint, W, Schuijs, M.J, Vanhoutte, L, Killeen, N, Hammad, H, & Lambrecht, B.N.M. (2018). The generation and use of allergen-specific TCR transgenic animals. In Methods in Molecular Biology. doi:10.1007/978-1-4939-7896-0_15