2019
Rectal Organoids Enable Personalized Treatment of Cystic Fibrosis
Publication
Publication
Cell Reports , Volume 26 - Issue 7 p. 1701- +
In vitro drug tests using patient-derived stem cell cultures offer opportunities to individually select efficacious treatments. Here, we provide a study that demonstrates that in vitro drug responses in rectal organoids from individual patients with cystic fibrosis (CF) correlate with changes in two in vivo therapeutic endpoints. We measured individual in vitro efficaciousness using a functional assay in rectum-derived organoids based on forskolininduced swelling and studied the correlation with in vivo effects. The in vitro organoid responses correlated with both change in pulmonary response and change in sweat chloride concentration. Receiver operating characteristic curves indicated good-toexcellent accuracy of the organoid-based test for defining clinical responses. This study indicates that an in vitro assay using stem cell cultures can prospectively select efficacious treatments for patients and suggests that biobanked stem cell resources can be used to tailor individual treatments in a cost-effective and patient-friendly manner.
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| doi.org/10.1016/j.celrep.2019.01.068, hdl.handle.net/1765/115231 | |
| Cell Reports | |
| Organisation | Department of Pediatrics |
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Berkers, G, van Mourik, P., Vonk, AM, Kruisselbrink, E, Dekkers, J., de Winter-de Groot, KM, … van der Ent, C. (2019). Rectal Organoids Enable Personalized Treatment of Cystic Fibrosis. Cell Reports, 26(7), 1701–+. doi:10.1016/j.celrep.2019.01.068 |
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