2006-04-05
Multiplex ligation-dependent probe amplification for the detection of 1p and 19q chromosomal loss in oligodendroglial tumors
Publication
Publication
Brain Pathology , Volume 15 - Issue 3 p. 192- 197
Multiplex ligation-dependent probe amplification (MLPA) is a new assay for the detection
of multiple chromosomal deletions in tumor tissue in a single experiment. Since
genotyping of gliomas with oligodendroglial features by the detection of 1p/19q chromosomal
deletions became essential for treatment decisions, we developed and validated
an MLPA- based assay to determine these losses in formalin fixed and paraffin
embedded oligodendroglial tumors (OG). Nineteen OGs, and 10 control samples were
analyzed by MLPA and the results were correlated with those obtained by fluorescent
in situ hybridization (FISH).
The MLPA results were reproducible in all samples in which
repeated experiments were performed. In 18 of 19 OGs, MLPA and FISH were concordant
for presence or absence of 1p deletion. In 3 OGs, MLPA detected a 19q deletion not
shown by FISH. For the other 15 OGs, MLPA and FISH were concordant. In one sample
with 50% to 75% of tumor, MLPA failed to detect the 1p/19q deletions revealed by FISH
(though with borderline values of significance). We conclude that MLPA is a valid and
reproducible method for the detection of 1p/19q chromosomal deletions in OGs stored
on formalin fixed, paraffin embedded tissue
Additional Metadata | |
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doi.org/10.1111/j.1750-3639.2005.tb00520.x, hdl.handle.net/1765/116352 | |
Brain Pathology | |
Organisation | Department of Virology |
Natté, R., van Eijk, R., Eilers, P., Cleton-Jansen, A.-M., Oosting, J., Kouwenhoven, M., … van Duinen, S. (2006). Multiplex ligation-dependent probe amplification for the detection of 1p and 19q chromosomal loss in oligodendroglial tumors. Brain Pathology, 15(3), 192–197. doi:10.1111/j.1750-3639.2005.tb00520.x |