The aim of the thesis, “Towards improved bone regeneration,” was to investigate ways to improve tissue engineered endochondral bone grafts. In this thesis we improve the construction of these grafts by identifying a more stable cell source with more favorable in vitro expansion characteristics compared to current gold standard cells, and expand upon our understanding of how such constructs form bone in vivo by investigating the importance of collagen type X during this process. By creating an easily injectable micropellet construct, an injectable endochondral bone forming construct was developed which could be used in combinational therapies with compounds, such as Emdogain, to perhaps further improve the effectiveness of graft outcomes which are described in more detail below. Together the studies in this thesis move our understanding and possible clinical applications for endochondral bone grafts forward.

Additional Metadata
Keywords Endochondral ossification, bone tissue engineering, mesenchymal stem cells, paediatric MSCs, collagen type x, micropellets, bone regeneration, msc expansion, chondrogenic differentiation
Promotor E.B. Wolvius (Eppo) , E. Farrell (Eric) , R. Narcisi (Roberto)
Publisher Erasmus University Rotterdam
ISBN 978-94-6361-275-3
Persistent URL hdl.handle.net/1765/117367
Note For copyright reasons there is a partial embargo for this dissertation
Citation
Knuth, C.A. (2019, June 25). Towards Improved Bone Regeneration. Erasmus University Rotterdam. Retrieved from http://hdl.handle.net/1765/117367