Introduction: Development of clinical biomarkers to guide therapy is an important unmet need in aneurysmal subarachnoid hemorrhage (SAH). A wide spectrum of plausible biomarkers has been reported for SAH, but none have been validated due to signifcant variabilities in study design, methodology, laboratory techniques, and outcome endpoints. Methods: A systematic review of SAH biomarkers was performed per the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. The panel’s recommendations focused on harmonization of (1) tar‑ get cellular and molecular biomarkers for future investigation in SAH, (2) standardization of best-practice procedures in biospecimen and biomarker studies, and (3) experimental method reporting requirements to facilitate meta-analy‑ ses and future validation of putative biomarkers. Results: No cellular or molecular biomarker has been validated for inclusion as “core” recommendation. Fifty-four stud‑ ies met inclusion criteria and generated 33 supplemental and emerging biomarker targets. Core recommendations include best-practice protocols for biospecimen collection and handling as well as standardized reporting guidelines to capture the heterogeneity and variabilities in experimental methodologies and biomarker analyses platforms. Conclusion: Signifcant variabilities in study design, methodology, laboratory techniques, and outcome endpoints exist in SAH biomarker studies and present signifcant barriers toward validation and translation of putative bio‑ markers to clinical use. Adaptation of common data elements, recommended biospecimen protocols, and report‑ ing guidelines will reduce heterogeneity and facilitate future meta-analyses and development of validated clinical biomarkers in SAH.

Additional Metadata
Keywords Biomarkers, Biospecimens, Cerebrospinal fuid, Subarachnoid hemorrhage, S100β, Tumor necrosis factor alpha, interleukin-6, metalloproteinase-9, Plasma-type gelsolin, Apolipoprotein E, Cardiac troponin I, B-type natriuretic peptide, common data elements, Standard operating procedure
Persistent URL,
Journal Neurocritical Care
Chou, S.H.Y., Macdonald, RL, Keller, E., Suarez, J.I., Macdonald, R.L.A., & Amin-Hanjani, S. (2019). Biospecimens and Molecular and Cellular Biomarkers in Aneurysmal Subarachnoid Hemorrhage Studies: Common Data Elements and Standard Reporting Recommendations. Neurocritical Care, 30, 46–59. doi:10.1007/s12028-019-00725-4