A better characterization of the HIV reservoir is pivotal for the development of effective eradication strategies. Accurate quantification of the latent reservoir remains challenging. Starting from a regular blood draw, the Tat/Rev induced limiting dilution assay (TILDA) combines serial dilution of CD4+ T cells with a PCR-based detection of HIV-1 spliced mRNA produced upon cell stimulation. Here we adapted the original protocol for HIV-1 subtype B to detect tat/rev mRNAs transcribed from reactivated latently infected cells in long term suppressed patients infected with HIV-1 subtype C. Given the heterogeneity of global HIV epidemiology, it is pivotal to develop assays with optimal performances also in patients infected with non-B subtypes. We observed that, in these patients infected with subtype C virus, the HIV reservoir quantified by TILDA correlates with both the time of virological suppression and CD4/CD8 ratio.

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doi.org/10.1016/j.jviromet.2019.113778, hdl.handle.net/1765/121773
Journal of Virological Methods
Department of Internal Medicine

Bertoldi, A., D'Urbano, V. (Vanessa), Bon, I., Verbon, A., Rokx, C., Boucher, C. (Charles), … Re, M.C. (Maria Carla). (2020). Development of C-TILDA: A modified TILDA method for reservoir quantification in long term treated patients infected with subtype C HIV-1. Journal of Virological Methods, 276. doi:10.1016/j.jviromet.2019.113778