2019-12-03
Characterization of the ferret TRB locus guided by V, D, J, and C gene expression analysis
Publication
Publication
The domestic ferret, Mustela putorius furo, is an important mammalian animal model to study human respiratory infection. However, insufficient genomic annotation hampers detailed studies of ferret T cell responses. In this study, we analyzed the published T cell receptor beta (TRB) locus and performed high-throughput sequencing (HTS) of peripheral blood of four healthy adult ferrets to identify expressed V, D, J, and C genes. The HTS data is used as a guide to manually curate the expressed V, D, J, and C genes. The ferret locus appears to be most similar to that of the dog. Like other mammalian TRB loci, the ferret TRB locus contains a library of variable genes located upstream of two D-J-C gene clusters, followed by a (in the ferret non-functional) V gene with an inverted transcriptional orientation. All TRB genes (expressed or not) reported here have been approved by the IMGT/WHO-IUIS nomenclature committee.
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| doi.org/10.1007/s00251-019-01142-9, hdl.handle.net/1765/122748 | |
| Immunogenetics | |
| Organisation | Department of Virology |
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Gerritsen, B. (Bram), Pandit, A. (Aridaman), Zaaraoui-Boutahar, F., van den hout, M., van IJcken, W., de Boer, R., & Andeweg, A. (2019). Characterization of the ferret TRB locus guided by V, D, J, and C gene expression analysis. Immunogenetics. doi:10.1007/s00251-019-01142-9 |
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